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 Scientists from AAFC-Saskatoon, AAFC-London, AAFC-Ottawa and from NRC-Saskatoon are focusing on seed development and composition in Brassica species and
Arabidopsis. The scientific objectives of the project are as
follows:
Seed development
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Functional Genomics of seed early stages development (R. Datla, W. Keller, D. Palmer and F. Georges)
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Functional Genomics of seed maturation and germination stages (A. Cutler and E. Tsang)
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Functional Genomics of microspore-derived embryos (J. Krochko and A.
Ferrie)
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Functional Genomics of seed coat development (G. Selvaraj)
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The role of LEA genes in late seed development and desiccation. (J. Singh and S. Abrams)
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Metabolic profiling of plant hormones of Brassica seed and embryo (S. Abrams)
Seed composition
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Characterization of genes involved in starch biosynthesis (J. Zou)
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Study of genes involved in lipid biosynthesis using Arabidopsis mutants (D. Taylor and D. Palmer)
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Regulation of storage protein Genes in Arabidopsis and Brassica using GFP proteins (Y. Cui and E. Tsang)
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Characterization of dehydrin, cruciferin and napin gene families in Arabidopsis and Brassica (D. Lydiate and A. Sharpe)
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Study of seed storage protein gene expression using epitope-tagged proteins (D. Hegedus and K. Rozwadowski)
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Identification of phenylpropanoid and phytate genes in mutant lines of Arabidopsis (A. Hannoufa)
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Seed metabolic profiling using the Synchrotron (M. Reaney)
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Generation of Arabidopsis homozygous lines and end sequencing DNA insertions from homozygous lines (I. Parkin and D. Lydiate)
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Fingerprinting of B. napus BAC clones (I. Parkin)
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