cDNA Array
The Brassica cDNA array was developed from 67,535 ESTs predominately from Brassica seeds. The analysis of these sequences identified 10,642 unigenes that were used for the preparation of a targeted seed cDNA array. A set of 10,642 PCR primer pairs was designed and corresponding amplicons produced for spotting, along with relevant controls. Critical quality control tests produced satisfactory results for use of these slides in biological experiments.
Number of genes/unique genes
10,642 unigenes prepared from 67,535 ESTs predominately from Brassica seeds.
GAL file and sequence information
Quality Control
60 slides are made per batch. One slide is used for quality control using DNA paragon staining.
Additional Information
A manuscript describing the Brassica cDNA array is in review (Xiang et al, Development of a Brasica seed cDNA microarray)
For additional information, contact Faouzi Bekkaoui.